04545nam a22006375i 4500 978-3-031-51171-4 DE-He213 20251030112021.0 cr nn 008mamaa 240430s2024 sz | s |||| 0|eng d 9783031511714 978-3-031-51171-4 10.1007/978-3-031-51171-4 doi QH506 QP801.P64 PSD bicssc PSBC bicssc SCI008000 bisacsh PSD thema Cryo-Electron Tomography [electronic resource] : Structural Biology in situ / edited by Friedrich Förster, Ariane Briegel. 1st ed. 2024. Cham : Springer International Publishing : Imprint: Springer, 2024. VIII, 389 p. 92 illus., 78 illus. in color. online resource. text txt rdacontent computer c rdamedia online resource cr rdacarrier text file PDF rda Focus on Structural Biology, 2542-9566 ; 11 1. Fundamentals of Instrumentation and electron optics For cryo-electron tomography -- 2. Principles of tomographic reconstruction -- 3. Cryo-preparation for cellular tomography -- 4. Cryo-FIB milling -- 5. In Situ Cryo-Electron Tomography and Advanced Micromanipulator Techniques -- 6. Labeling approaches and correlation with light microscopy (Cryo) CLEM -- 7. Data collection -- 8. Subvolume averaging and classification -- 9. High resolution tomography/ Molecular model building and visualization -- 10. Beam-induced Motion Mechanism and Correction for Improved Cryo-Electron Microscopy and Cryo-Electron Tomography -- 11. Tomogram segmentation -- 12. Electron tomography of cryo-fixed and resin-embedded samples -- 13. Democratization of tomographic data / FAIR data. This book presents key aspects and recent developments of cryogenic sample electron tomography (cryo-ET) methodology, authored by leading experts in the field. Understanding structure and function of biomolecules in the context of cells is a new frontier in cellular and structural biology. To facilitate such research, cryo-ET is a key method to visualize the molecules of life in their native settings. Cryo-ET enables the imaging of samples that are preserved in a near-native state, at (macro)-molecular resolution and in three dimensions. Thus, this technique is a unique tool to gain insights into how biomolecules collaborate in orchestrating fundamental biological processes, how mutations cause diseases, pathogens cause infections, and to develop novel therapeutics to treat such illnesses. This book provides a unique reference for the emerging field of cryo-ET. The topics covered range from the fundamental principles of imaging to sample preparation, data analysis, and data sharing within the scientific community. It serves as a valuable resource for the next generation of structural biologists, making it suitable both for undergraduate students studying biochemistry, biophysics, and molecular biology and highly valuable for the more experienced and specialized PhD student. Furthermore, it stands as a state-of-the–art source of knowledge for the established senior scientist within the field of structural biology. Biomolecules. 66771 Physical biochemistry. 76108 Macromolecules. 76109 Biology Technique. 67845 Biophysics. 66239 Immunology. 69214 Materials Microscopy. 69162 Structural Biology. 76110 Biophysical Methods. 76111 Immunology. 69214 Microscopy. 69164 Förster, Friedrich. editor. edt http://id.loc.gov/vocabulary/relators/edt 76112 Briegel, Ariane. editor. edt http://id.loc.gov/vocabulary/relators/edt 76113 SpringerLink (Online service) Springer Nature eBook Printed edition: 9783031511707 Printed edition: 9783031511721 Printed edition: 9783031511738 Focus on Structural Biology, 2542-9566 ; 11 76114 https://doi.org/10.1007/978-3-031-51171-4 ZDB-2-SBL ZDB-2-SXB 59540 59540