04328nam a22005895i 4500001001800000003000900018005001700027007001500044008004100059020003700100024003500137050001000172050001400182072001600196072001700212072002300229072001500252245013200267250001800399264007500417300006600492336002600558337002600584338003600610347002400646490005000670505077800720520144301498650002502941650003402966650002703000650003103027650002303058650002303081650003403104650003103138650003203169650002303201650002303224700009003247700008503337710003403422773002603456776003603482776003603518776003603554830005703590856004603647912001403693912001403707999001703721978-3-031-51171-4DE-He21320251030112021.0cr nn 008mamaa240430s2024    sz |    s    |||| 0|eng d  a97830315117149978-3-031-51171-47 a10.1007/978-3-031-51171-42doi 4aQH506 4aQP801.P64 7aPSD2bicssc 7aPSBC2bicssc 7aSCI0080002bisacsh 7aPSD2thema10aCryo-Electron Tomographyh[electronic resource] :bStructural Biology in situ /cedited by Friedrich Förster, Ariane Briegel.  a1st ed. 2024. 1aCham :bSpringer International Publishing :bImprint: Springer,c2024.  aVIII, 389 p. 92 illus., 78 illus. in color.bonline resource.  atextbtxt2rdacontent  acomputerbc2rdamedia  aonline resourcebcr2rdacarrier  atext filebPDF2rda1 aFocus on Structural Biology,x2542-9566 ;v110 a1. Fundamentals of Instrumentation and electron optics For cryo-electron tomography -- 2. Principles of tomographic reconstruction  -- 3. Cryo-preparation for cellular tomography -- 4. Cryo-FIB milling -- 5. In Situ Cryo-Electron Tomography and Advanced Micromanipulator Techniques -- 6. Labeling approaches and correlation with light microscopy (Cryo) CLEM -- 7. Data collection -- 8. Subvolume averaging and classification -- 9. High resolution tomography/ Molecular model building and visualization -- 10. Beam-induced Motion Mechanism and Correction for Improved Cryo-Electron Microscopy and Cryo-Electron Tomography -- 11. Tomogram segmentation -- 12. Electron tomography of cryo-fixed and resin-embedded samples -- 13. Democratization of tomographic data / FAIR data.  aThis book presents key aspects and recent developments of cryogenic sample electron tomography (cryo-ET) methodology, authored by leading experts in the field. Understanding structure and function of biomolecules in the context of cells is a new frontier in cellular and structural biology. To facilitate such research, cryo-ET is a key method to visualize the molecules of life in their native settings. Cryo-ET enables the imaging of samples that are preserved in a near-native state, at (macro)-molecular resolution and in three dimensions. Thus, this technique is a unique tool to gain insights into how biomolecules collaborate in orchestrating fundamental biological processes, how mutations cause diseases, pathogens cause infections, and to develop novel therapeutics to treat such illnesses. This book provides a unique reference for the emerging field of cryo-ET. The topics covered range from the fundamental principles of imaging to sample preparation, data analysis, and data sharing within the scientific community. It serves as a valuable resource for the next generation of structural biologists, making it suitable both for undergraduate students studying biochemistry, biophysics, and molecular biology and highly valuable for the more experienced and specialized PhD student. Furthermore, it stands as a state-of-the–art source of knowledge for the established senior scientist within the field of structural biology. 0aBiomolecules.966771 0aPhysical biochemistry.976108 0aMacromolecules.976109 0aBiologyxTechnique.967845 0aBiophysics.966239 0aImmunology.969214 0aMaterialsxMicroscopy.96916214aStructural Biology.97611024aBiophysical Methods.97611124aImmunology.96921424aMicroscopy.9691641 aFörster, Friedrich.eeditor.4edt4http://id.loc.gov/vocabulary/relators/edt9761121 aBriegel, Ariane.eeditor.4edt4http://id.loc.gov/vocabulary/relators/edt9761132 aSpringerLink (Online service)0 tSpringer Nature eBook08iPrinted edition:z978303151170708iPrinted edition:z978303151172108iPrinted edition:z9783031511738 0aFocus on Structural Biology,x2542-9566 ;v1197611440uhttps://doi.org/10.1007/978-3-031-51171-4  aZDB-2-SBL  aZDB-2-SXB  c59540d59540