04240nam a22004695i 4500001001800000003000900018005001700027007001500044008004100059020003700100024003500137050001400172072001600186072002300202072001500225245010200240250001800342264007200360300006700432336002600499337002600525338003600551347002400587505048500611520215801096650002503254650003303279650002703312650002503339650003303364650002603397700008803423710003403511773002603545776003603571776003603607776003603643856004603679912001403725912001403739999001703753978-981-97-6851-6DE-He21320251030114651.0cr nn 008mamaa241230s2024    si |    s    |||| 0|eng d  a97898197685169978-981-97-6851-67 a10.1007/978-981-97-6851-62doi 4aRM300-666 7aMMG2bicssc 7aMED0710002bisacsh 7aMKG2thema10aAntibiotics and Their Mechanisms of Actionh[electronic resource] /cedited by Deepesh Nagarajan.  a1st ed. 2024. 1aSingapore :bSpringer Nature Singapore :bImprint: Springer,c2024.  aXIII, 177 p. 119 illus., 98 illus. in color.bonline resource.  atextbtxt2rdacontent  acomputerbc2rdamedia  aonline resourcebcr2rdacarrier  atext filebPDF2rda0 aChapter 1. The history of antibiotics -- Chapter 2. Methods for antibiotic discovery -- Chapter 3. Penicillin -- Chapter 4. Vancomycin -- Chapter 5. Antimicrobial peptides -- Chapter 6. Quinolones -- Chapter 7. Rifampicin -- Chapter 8. Tetracyclines -- Chapter 9. Streptomycin -- Chapter 10. Chloramphenicol -- Chapter 11. Antimetabolites -- Chapter 12. Mycolic acid inhibitors -- Chapter 13. Other antimicrobial agents -- Chapter 14. Antibiotic Pharmacology and Biopharmaceutics.  a“Antibiotics and their mechanisms of action” provides a deep and lucid understanding of the mechanisms of action of various antibiotics. The experiments followed to decipher the mechanisms have been explained in great depth, which is a novelty in this textbook. Most undergraduate textbooks on antibiotics place emphasis on their use from the clinician’s perspective. Taking penicillin as an example, typical textbooks discuss its discovery, penicillin’s β-lactam derivatives, routes of administration, the diseases and pathogens treated with β-lactam antibiotics, side effects, and finally the mechanisms of resistance that have evolved against penicillin. No description of how its mechanism of action was deciphered is ever provided. This textbook describes how simple experiments using protoplasts, covalent affinity chromatography, SYPRO Orange binding assays, and X-ray crystallography have all been used to elucidate the mechanism of action of penicillin. Like this, the complicated mechanisms of action of different antibiotics have been presented in simple terms with the help of original illustrations. Chapters 8-10 cover the mechanisms of action of anti-ribosomal antibiotics (tetracycline, streptomycin, chloramphenicol). Elucidating the mechanisms of action for these antibiotics requires complex experiments such as ex vivo transcription via a cell-free ribosomal reaction mixture, photolabelling experiments, and sucrose density gradient centrifugation of radiolabelled translation reactants and products. These experiments are conveyed to the reader in the simplest possible language and with easily understandable, appealing illustrations. Finally, the reader is challenged at the end of every chapter with original questions, testing whether hecan apply the concepts he learned in the preceding chapter to research-level problems. This book is written primarily for undergraduate students in microbiology or biotechnology courses to help them develop a simplistic yet comprehensive understanding of the mechanisms underlying antibiotics action. It is believed that this book will prepare the students for PhD level research. 0aPharmacology.967844 0aMedical microbiology.963858 0aDrug delivery systems.14aPharmacology.96784424aMedical Microbiology.96385824aDrug Delivery.9694291 aNagarajan, Deepesh.eeditor.4edt4http://id.loc.gov/vocabulary/relators/edt9899082 aSpringerLink (Online service)0 tSpringer Nature eBook08iPrinted edition:z978981976850908iPrinted edition:z978981976852308iPrinted edition:z978981976853040uhttps://doi.org/10.1007/978-981-97-6851-6  aZDB-2-SBL  aZDB-2-SXB  c66205d66205