03115nam a22003614u 450000100220000000300060002200500170002800700150004500800410006002000300010102400310013104100080016210000270017024501110019725000060030826000160031426400160033030000120034633600260035833700260038433800360041034700210044634700080046752016200047565000280209565000290212365000230215265302340217577600360240985600640244585601020250985601420261110.1002/9783527836840Wiley20251030120313.0cr nn 008mam|a240402t2024||||xxu    fs|   |0|| 0|eng||  a9783527836840qelectronic7 a10.1002/97835278368402doi  aeng1 aUchiyama, Seiichi4aut10aIntracellular Thermometry with Fluorescent Molecular Thermometersh[electronic resource]cSeiichi Uchiyama  a1  bWileyc2024 1bWileyc2024  a352pp.;  atextbtxt2rdacontent  acomputerbc2rdamedia  aonline resourcebcr2rdacarrier  atext file2rdaft  bPDF  aIntracellular Thermometry with Fluorescent Molecular Thermometers Understand a vital new bioanalytical technique with this comprehensive introduction to measuring temperature on the cellular scale Most organisms have highly controlled body temperatures, fluctuations in which are therefore sensitive indicators of changes in body function. In recent years, the development of fluorescent molecular thermometers and related intracellular temperature probes has enabled researchers to track these fluctuations at the cellular rather than the organismic level, opening up a whole new field of study in cell and molecular biology. Intracellular Thermometry with Fluorescent Molecular Thermometers provides bioanalytical researchers with an introduction to these technologies and their current and future applications. Starting off with a discussion of temperature as a key factor in biological regulation, it provides an authoritative overview of available fluorescent temperature probes, their characteristics and potential applications. Intracellular Thermometry with Fluorescent Molecular Thermometers readers will also find: Step by step instructions for constructing an intracellular thermometry experiment and validating results Comprehensive discussion of existing applications A vision for the future development of thermal biology as an independent discipline Authored by a pioneer in the field of intracellular thermometry, Intracellular Thermometry with Fluorescent Molecular Thermometers is ideal for researchers in analytical chemistry, cell biology, molecular biology, biophysics, or any related subjects. 4aBioanalytical Chemistry 4aCell & Molecular Biology 4aBiomedical Imaging00aBody temperature; temperature curve; biological functions; intracellular temperature probes; physiological monitoring; disease diagnosis; cellular temperature patterns; bioanalytical sciences; experimental design; thermal biology08iPrinted edition:z978352735032240uhttps://doi.org/10.1002/9783527836840aWiley Online Library40uhttps://onlinelibrary.wiley.com/action/showBook?doi=10.1002%2F9783527836840aWiley Online Library423Cover Imageuhttps://onlinelibrary.wiley.com/cms/asset/abe8f82f-dd65-4fc5-8094-1f9a27bed5d6/9783527836840.cover.gifaWiley Online Library