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020 _a9783031511714
_9978-3-031-51171-4
024 7 _a10.1007/978-3-031-51171-4
_2doi
050 4 _aQH506
050 4 _aQP801.P64
072 7 _aPSD
_2bicssc
072 7 _aPSBC
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072 7 _aSCI008000
_2bisacsh
072 7 _aPSD
_2thema
245 1 0 _aCryo-Electron Tomography
_h[electronic resource] :
_bStructural Biology in situ /
_cedited by Friedrich Förster, Ariane Briegel.
250 _a1st ed. 2024.
264 1 _aCham :
_bSpringer International Publishing :
_bImprint: Springer,
_c2024.
300 _aVIII, 389 p. 92 illus., 78 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aFocus on Structural Biology,
_x2542-9566 ;
_v11
505 0 _a1. Fundamentals of Instrumentation and electron optics For cryo-electron tomography -- 2. Principles of tomographic reconstruction -- 3. Cryo-preparation for cellular tomography -- 4. Cryo-FIB milling -- 5. In Situ Cryo-Electron Tomography and Advanced Micromanipulator Techniques -- 6. Labeling approaches and correlation with light microscopy (Cryo) CLEM -- 7. Data collection -- 8. Subvolume averaging and classification -- 9. High resolution tomography/ Molecular model building and visualization -- 10. Beam-induced Motion Mechanism and Correction for Improved Cryo-Electron Microscopy and Cryo-Electron Tomography -- 11. Tomogram segmentation -- 12. Electron tomography of cryo-fixed and resin-embedded samples -- 13. Democratization of tomographic data / FAIR data.
520 _aThis book presents key aspects and recent developments of cryogenic sample electron tomography (cryo-ET) methodology, authored by leading experts in the field. Understanding structure and function of biomolecules in the context of cells is a new frontier in cellular and structural biology. To facilitate such research, cryo-ET is a key method to visualize the molecules of life in their native settings. Cryo-ET enables the imaging of samples that are preserved in a near-native state, at (macro)-molecular resolution and in three dimensions. Thus, this technique is a unique tool to gain insights into how biomolecules collaborate in orchestrating fundamental biological processes, how mutations cause diseases, pathogens cause infections, and to develop novel therapeutics to treat such illnesses. This book provides a unique reference for the emerging field of cryo-ET. The topics covered range from the fundamental principles of imaging to sample preparation, data analysis, and data sharing within the scientific community. It serves as a valuable resource for the next generation of structural biologists, making it suitable both for undergraduate students studying biochemistry, biophysics, and molecular biology and highly valuable for the more experienced and specialized PhD student. Furthermore, it stands as a state-of-the–art source of knowledge for the established senior scientist within the field of structural biology.
650 0 _aBiomolecules.
_966771
650 0 _aPhysical biochemistry.
_976108
650 0 _aMacromolecules.
_976109
650 0 _aBiology
_xTechnique.
_967845
650 0 _aBiophysics.
_966239
650 0 _aImmunology.
_969214
650 0 _aMaterials
_xMicroscopy.
_969162
650 1 4 _aStructural Biology.
_976110
650 2 4 _aBiophysical Methods.
_976111
650 2 4 _aImmunology.
_969214
650 2 4 _aMicroscopy.
_969164
700 1 _aFörster, Friedrich.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
_976112
700 1 _aBriegel, Ariane.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
_976113
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9783031511707
776 0 8 _iPrinted edition:
_z9783031511721
776 0 8 _iPrinted edition:
_z9783031511738
830 0 _aFocus on Structural Biology,
_x2542-9566 ;
_v11
_976114
856 4 0 _uhttps://doi.org/10.1007/978-3-031-51171-4
912 _aZDB-2-SBL
912 _aZDB-2-SXB
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